Leishmaniasis research by parasitological lymph node examination, PCR and species characterization by RFLP-PCR in municipal shelter dogs previously submitted to immunochromatographic testing
DOI:
https://doi.org/10.36557/2009-3578.2025v11n2p2275-2298Keywords:
single health, epidemiological surveillance, chitinase gene, animal shelterAbstract
This study investigated Leishmania spp. infection in 153 dogs from a municipal shelter in an endemic area, previously tested as non-reactive by immunochromatography. Parasitological lymph node examination and PCR-RFLP directed to the chitinase gene were used for diagnosis and characterization of the species. The parasitological examination detected amastigotes in 3.3% of the dogs, while the PCR confirmed the infection in 1.3%, with high agreement between the methods (98%; Kappa=0.563; p<0.001). RFLP analysis identified the species as Leishmania infantum chagasi. The presence of clinical signs was not significantly associated with positive results. The results demonstrate the circulation of the parasite even in dogs with negative serological screening, evidencing the limitation of the isolated use of rapid tests and the need to adopt combined and more sensitive diagnostic protocols in shelters in endemic areas for the effective control of Canine Visceral Leishmaniasis.
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Copyright (c) 2025 Élida de Oliveira Ribeiro , Rodrigo Buzinaro Suzuki, Caio Ferreira Repik, Raquel Richter Nazari, Márcia Aparecida Sperança, Isabela Bazzo da Costa, Patricia Cincotto dos Santos Bueno, Rodrigo Prevedello Franco, Camila Dias Porto
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